Robust polarity establishment occurs via an endocytosis-based cortical corralling mechanism
J Cell Biol. 2013-02-11; 200(4): 407-418
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1. J Cell Biol. 2013 Feb 18;200(4):407-18. doi: 10.1083/jcb.201206081. Epub 2013 Feb
Robust polarity establishment occurs via an endocytosis-based cortical corralling
Jose M(1), Tollis S, Nair D, Sibarita JB, McCusker D.
(1)Dynamics of Cell Growth and Division, Institut de Biologie Cellulaire et de
Génétique, Centre National de la Recherche Scientifique, UMR 5095, 33000
J Cell Biol. 2013 Feb 18;200(4):367-71.
Formation of a stable polarity axis underlies numerous biological processes.
Here, using high-resolution imaging and complementary mathematical modeling we
find that cell polarity can be established via the spatial coordination of
opposing membrane trafficking activities: endocytosis and exocytosis. During
polarity establishment in budding yeast, these antagonistic processes become
apposed. Endocytic vesicles corral a central exocytic zone, tightening it to a
vertex that establishes the polarity axis for the ensuing cell cycle.
Concomitantly, the endocytic system reaches an equilibrium where internalization
events occur at a constant frequency. Endocytic mutants that failed to initiate
periodic internalization events within the corral displayed wide, unstable
polarity axes. These results, predicted by in silico modeling and verified by
high resolution in vivo studies, identify a requirement for endocytic corralling
during robust polarity establishment.
PMID: 23401000 [Indexed for MEDLINE]