Quantitative evaluation of ultrasound-mediated cellular uptake of a fluorescent model drug.
Mol Imaging Biol. 2013-03-12; 15(5): 523-533
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1. Mol Imaging Biol. 2013 Oct;15(5):523-33. doi: 10.1007/s11307-013-0615-1.
Quantitative evaluation of ultrasound-mediated cellular uptake of a fluorescent
Lepetit-Coiffé M(1), Yudina A, Poujol C, de Oliveira PL, Couillaud F, Moonen CT.
(1)Laboratoire IMF UMR 5231 CNRS, Université Bordeaux 2, Bordeaux, France,
PURPOSE: This study aims to quantitatively analyze cellular uptake following
local ultrasound (US)-mediated cell permeabilization.
PROCEDURES: A 2 μM cell-impermeable dye Sytox Green was co-injected with
3 × 10(7) microbubbles in the presence of C6 rat glioblastoma cell monolayer in
total volume of 10 ml. A 5.8-mm diameter mono-element US transducer was
positioned at a distance of 8 mm to the Opticell® membrane. Acoustical pressure
of pulsed US was varied from 0.62 MPa peak-to-peak (p-p) to 1.25 MPa p-p. Large
field of view (FOV = 15 × 15 mm) 22 × 22 mosaic acquisitions were done under
epifluorescence Leica DMR microscope and analyzed in Metamorph software to
evaluate cell density as well as model drug uptake percentage.
RESULTS: The size of acoustical field of the transducer closely matches the
spatial pattern of the model drug internalized into the cells by US. Maximum of
uptake percentage (42 ± 15 %) was found at 0.88 MPa p-p.
CONCLUSIONS: Spatial aspect of US-mediated model drug uptake has been
quantitatively evaluated on adherent cells using robust 2D-mapping approach.
PMID: 23479324 [Indexed for MEDLINE]