Proteomic Analysis of Gliosomes from Mouse Brain: Identification and Investigation of Glial Membrane Proteins
J. Proteome Res.. 2014-11-04; 13(12): 5918-5927
DOI: 10.1021/pr500829z
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1. J Proteome Res. 2014 Dec 5;13(12):5918-27. doi: 10.1021/pr500829z. Epub 2014 Nov
4.
Proteomic analysis of gliosomes from mouse brain: identification and
investigation of glial membrane proteins.
Carney KE(1), Milanese M, van Nierop P, Li KW, Oliet SH, Smit AB, Bonanno G,
Verheijen MH.
Author information:
(1)Department of Molecular & Cellular Neurobiology, Center for Neurogenomics and
Cognitive Research, Neuroscience Campus Amsterdam, VU University Amsterdam , 1081
HV Amsterdam, The Netherlands.
Astrocytes are being increasingly recognized as crucial contributors to neuronal
function at synapses, axons, and somas. Reliable methods that can provide insight
into astrocyte proteins at the neuron-astrocyte functional interface are highly
desirable. Here, we conducted a mass spectrometry analysis of Percoll
gradient-isolated gliosomes, a viable preparation of glial subcellular particles
often used to study mechanisms of astrocytic transmitter uptake and release and
their regulation. Gliosomes were compared with synaptosomes, a preparation
containing the neurotransmitter release machinery, and, accordingly, synaptosomes
were enriched for proteins involved in synaptic vesicle-mediated transport.
Interestingly, gliosome preparations were found to be enriched for different
classes of known astrocyte proteins, such as VAMP3 (involved in astrocyte
exocytosis), Ezrin (perisynaptic astrocyte cytoskeletal protein), and Basigin
(astrocyte membrane glycoprotein), as well as for G-protein-mediated signaling
proteins. Mass spectrometry data are available via ProteomeXchange with the
identifier PXD001375. Together, these data provide the first detailed description
of the gliosome proteome and show that gliosomes can be a useful preparation to
study glial membrane proteins and associated processes.
DOI: 10.1021/pr500829z
PMID: 25308431 [Indexed for MEDLINE]