Peptidergic paracrine and endocrine cells in the midgut of the fruit fly maggot
Cell Tissue Res. 2009-03-25; 336(2): 309-323
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1. Cell Tissue Res. 2009 May;336(2):309-23. doi: 10.1007/s00441-009-0769-y. Epub
2009 Mar 25.
Peptidergic paracrine and endocrine cells in the midgut of the fruit fly maggot.
(1)Université de Bordeaux, CNIC CNRS UMR 5228, Talence, France.
Endocrine cells in the larval midgut of Drosophila melanogaster are recognized by
antisera to seven regulatory peptides: the allatostatins A, B, and C, short
neuropeptide F, neuropeptide F, diuretic hormone 31, and the tachykinins. These
are the same peptides that are produced by the endocrine cells of the adult
midgut, except for short neuropeptide F, which is absent in adult midgut
endocrine cells. The anterior larval midgut contains two types of endocrine
cells. The first produces short neuropeptide F, which is also recognized by an
antiserum to the receptor for the diuretic hormone leucokinin. The second type in
the anterior midgut is recognized by an antiserum to diuretic hormone 31. The
latter cell type is also found in the junction between the anterior and middle
midgut; an additional type of endocrine cell in this region produces allatostatin
B, a peptide also known as myoinhibitory peptide. Both types of endocrine cells
in the junction between the anterior and middle midgut can express the
homeodomain transcription factor labial. The copper cell region contains small
cells that either produce allatostatin C or a combination of neuropeptide F,
allatostatin B, and diuretic hormone 31. The latter cell type is also found in
the region of the large flat cells. The posterior midgut possesses strongly
immunoreactive allatostatin C endocrine cells immediately behind the iron cells.
In the next part of the posterior midgut, two cell types have been found: one
produces diuretic hormone 31, and a second is strongly immunoreactive to
antiserum against the leucokinin receptor and weakly immunoreactive to antisera
against allatostatins B and C and short neuropeptide F. The last part of the
posterior midgut again has two types of endocrine cells: those that produce
allatostatin A, and those that produce tachykinins. Many of the latter cells are
also weakly immunoreactive to the antiserum against diuretic hormone 31. As in
the adult, the insulin-like peptide 3 gene appears to be expressed by midgut
muscles, but not by midgut endocrine cells.
PMID: 19319573 [Indexed for MEDLINE]