Organic material concentration in auditory outer hair cells measured by laser interferometry.
Cytometry. 1995-05-01; 20(1): 1-6
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1. Cytometry. 1995 May 1;20(1):1-6.
Organic material concentration in auditory outer hair cells measured by laser
Laffon E(1), Dilhaire S, Aurousseau C, Dulon D, Claeys W.
(1)Laboratoire d’Audiologie Expérimentale, Université de Bordeaux II, Hôpital
Cytometry 1995 Jul 1;20(3):272.
Outer hair cells (OHC) of the mammalian cochlea are quasicylindrical cells of
different length, which play a major role in hearing at threshold. Their
particular shape allows the use of a noninvasive laser interferometric technique
of isolated cells in vitro in order to measure the organic material concentration
(OMC), hence the density of each cell body. In most (95%) of the OHCs isolated
from the same guinea pig, when the cell diameter is normalized, the results show
that the cell body OMC does not vary with cell length. In different animals, the
respective normalized OMC mean values can vary between 70 kg/m3 and 103 kg/m3. A
few OHCs with morphological particularities often possess cell body OMCs > 103
kg/m3. The results of the interferometric measurements in isolated OHCs confirm
that density variations in the cell bodies are not involved in a sound frequency
coding. The in vitro OMC variations of the OHCs could be related to the isolation
procedure; however, they could also correlate with actual in vivo OMC variations.
PMID: 7600894 [Indexed for MEDLINE]