Object recognition test in mice.
Nat Protoc. 2013-11-21; 8(12): 2531-2537
DOI: 10.1038/nprot.2013.155
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1. Nat Protoc. 2013 Dec;8(12):2531-7. doi: 10.1038/nprot.2013.155. Epub 2013 Nov 21.
Object recognition test in mice.
Leger M(1), Quiedeville A, Bouet V, Haelewyn B, Boulouard M, Schumann-Bard P,
Freret T.
Author information:
(1)1] Groupe Mémoire et Plasticité comportementale (GMPc), Université de Caen
Basse-Normandie, Caen, France. [2].
The object recognition test is now among the most commonly used behavioral tests
for mice. A mouse is presented with two similar objects during the first session,
and then one of the two objects is replaced by a new object during a second
session. The amount of time taken to explore the new object provides an index of
recognition memory. As more groups have used the protocol, the variability of the
procedures used in the object recognition test has increased steadily. This
protocol provides a necessary standardization of the procedure. This protocol
reduces inter-individual variability with the use of a selection criterion based
on a minimal time of exploration for both objects during each session. In this
protocol, we describe the three most commonly used variants, containing long (3
d), short (1 d) or no habituation phases. Thus, with a short intersession
interval (e.g., 6 h), this procedure can be performed in 4, 2 or 1 d,
respectively, according to the duration of the habituation phase. This protocol
should allow for the comparison of results from different studies, while
permitting adaption of the protocol to the constraints of the experimenter.
DOI: 10.1038/nprot.2013.155
PMID: 24263092 [Indexed for MEDLINE]