Mirror slides for high-sensitivity cell and tissue fluorescence imaging.
J. Biomed. Opt.. 2007-01-01; 12(2): 024030
DOI: 10.1117/1.2722730
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1. J Biomed Opt. 2007 Mar-Apr;12(2):024030.
Mirror slides for high-sensitivity cell and tissue fluorescence imaging.
Le Moal E(1), Fort E, Léve Que-Fort S, Janin A, Murata H, Cordelières FP,
Fontaine-Aupart MP, Ricolleau C.
Author information:
(1)Université Denis Diderot – Paris VII, UMR 7162 CNRS, Laboratoire Matériaux et
Phénomènes Quantiques, Paris Cedex, France.
Fluorescence microscopy has become the method of choice in the majority of
life-science applications. We describe development and use of mirror slides to
significantly enhance the fluorescence signal using standard air microscope
objectives. This technique offers sufficient gain to achieve high-sensitivity
imaging, together with wide field of observation and large depth of focus, two
major breakthroughs for routine analysis and high-throughput screening
applications on cells and tissue samples.
DOI: 10.1117/1.2722730
PMID: 17477745 [Indexed for MEDLINE]