Leucokinin and diuretic hormone immunoreactivity of neurons in the tobacco hornworm, Manduca sexta, and co-localization of this immunoreactivity in lateral neurosecretory cells of abdominal ganglia
Cell Tissue Res. 1994-12-01; 278(3): 493-507
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1. Cell Tissue Res. 1994 Dec;278(3):493-507.
Leucokinin and diuretic hormone immunoreactivity of neurons in the tobacco
hornworm, Manduca sexta, and co-localization of this immunoreactivity in lateral
neurosecretory cells of abdominal ganglia.
Chen Y(1), Veenstra JA, Hagedorn H, Davis NT.
(1)Center for Insect Science, University of Arizona, Tucson 85721.
Because leucokinins stimulate diuresis in some insects, we wished to identify the
neurosecretory cells in Manduca sexta that might be a source of leucokinin-like
neurohormones. Immunostaining was done at various stages of development, using an
antiserum to leucokinin IV. Bilateral pairs of neurosecretory cells in abdominal
ganglia 3-7 of larvae and adults are immunoreactive; these cells project via the
ipsilateral ventral nerves to the neurohemal transverse nerves. The
immunoreactivity and size of these lateral cells greatly increases in the pharate
adult, and this change appears to be related to a period of intensive diuresis
occurring a few days before adult eclosion. Relationships of these neurons to
cells that are immunoreactive to a M. sexta diuretic hormone were also
investigated. Diuretic hormone and leucokinin immunoreactivity are co-localized
in the lateral neurosecretory cells and their neurohemal projections. A median
pair of leucokinin-immunoreactive, and a lateral pair of diuretic
hormone-immunoreactive neurons in the larval terminal abdominal ganglion project
to neurohemal release sites within the cryptonephridium. The immunoreactivity of
these cells is lost as the cryptonephridium is eliminated during metamorphosis.
This loss appears to be related to the change from the larval to adult pattern of
PMID: 7850860 [Indexed for MEDLINE]