Large-scale imaging of cortical network activity with calcium indicators.

Yuji Ikegaya, Morgane Le Bon-Jego, Rafael Yuste
Neuroscience Research. 2005-06-01; 52(2): 132-138
DOI: 10.1016/j.neures.2005.02.004


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1. Neurosci Res. 2005 Jun;52(2):132-8. Epub 2005 Mar 17.

Large-scale imaging of cortical network activity with calcium indicators.

Ikegaya Y(1), Le Bon-Jego M, Yuste R.

Author information:
(1)Department of Biological Sciences, Columbia University, 1002 Fairchild Center,
M.C. 2435 New York, NY 10027, USA.

Bulk loading of calcium indicators has provided a unique opportunity to
reconstruct the activity of cortical networks with single-cell resolution. Here
we describe the detailed methods of bulk loading of AM dyes we developed and have
been improving for imaging with a spinning disk confocal microscope.

DOI: 10.1016/j.neures.2005.02.004
PMID: 15893573 [Indexed for MEDLINE]

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