In vivo optogenetic identification and manipulation of GABAergic interneuron subtypes.

Lisa Roux, Eran Stark, Lucas Sjulson, György Buzsáki
Current Opinion in Neurobiology. 2014-06-01; 26: 88-95
DOI: 10.1016/j.conb.2013.12.013

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1. Curr Opin Neurobiol. 2014 Jun;26:88-95. doi: 10.1016/j.conb.2013.12.013. Epub
2014 Jan 14.

In vivo optogenetic identification and manipulation of GABAergic interneuron
subtypes.

Roux L(1), Stark E(1), Sjulson L(2), Buzsáki G(3).

Author information:
(1)NYU Neuroscience Institute, School of Medicine and Center for Neural Science,
New York University, New York, NY 10016, United States.
(2)NYU Neuroscience Institute, School of Medicine and Center for Neural Science,
New York University, New York, NY 10016, United States; Department of Psychiatry,
New York University Medical Center, New York, NY 10016, United States.
(3)NYU Neuroscience Institute, School of Medicine and Center for Neural Science,
New York University, New York, NY 10016, United States. Electronic address:
.

Identification and manipulation of different GABAergic interneuron classes in the
behaving animal are important to understand their role in circuit dynamics and
behavior. The combination of optogenetics and large-scale neuronal recordings
allows specific interneuron populations to be identified and perturbed for
circuit analysis in intact animals. A crucial aspect of this approach is coupling
electrophysiological recording with spatially and temporally precise light
delivery. Focal multisite illumination of neuronal activators and silencers in
predetermined temporal configurations or a closed loop manner opens the door to
addressing many novel questions. Recent progress demonstrates the utility and
power of this novel technique for interneuron research.

Copyright © 2013 Elsevier Ltd. All rights reserved.

DOI: 10.1016/j.conb.2013.12.013
PMCID: PMC4024355
PMID: 24440414 [Indexed for MEDLINE]

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