In vivo imaging of intersynaptic vesicle exchange using VGLUT1 Venus knock-in mice.

E. Herzog, F. Nadrigny, K. Silm, C. Biesemann, I. Helling, T. Bersot, H. Steffens, R. Schwartzmann, U. V. Nagerl, S. El Mestikawy, J. Rhee, F. Kirchhoff, N. Brose
Journal of Neuroscience. 2011-10-26; 31(43): 15544-15559
DOI: 10.1523/JNEUROSCI.2073-11.2011

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1. J Neurosci. 2011 Oct 26;31(43):15544-59. doi: 10.1523/JNEUROSCI.2073-11.2011.

In vivo imaging of intersynaptic vesicle exchange using VGLUT1 Venus knock-in

Herzog E(1), Nadrigny F, Silm K, Biesemann C, Helling I, Bersot T, Steffens H,
Schwartzmann R, Nägerl UV, El Mestikawy S, Rhee J, Kirchhoff F, Brose N.

Author information:
(1)Department of Molecular Neurobiology, Max Planck Institute of Experimental
Medicine, D-37075 Göttingen, Germany.

Comment in
J Neurosci. 2012 Mar 7;32(10):3284-6.

The vesicular glutamate transporter VGLUT1 loads synaptic vesicles with the
neurotransmitter glutamate and thereby determines glutamate release at many
synapses in the mammalian brain. Due to its function and selective localization,
VGLUT1 is one of the most specific markers for glutamatergic synaptic vesicles.
It has been used widely to identify glutamatergic synapses, and its expression
levels are tightly correlated with changes in quantal size, modulations of
synaptic plasticity, and corresponding behaviors. We generated a fluorescent
VGLUT1(Venus) knock-in mouse for the analysis of VGLUT1 and glutamatergic
synaptic vesicle trafficking. The mutation does not affect glutamatergic synapse
function, and thus the new mouse model represents a universal tool for the
analysis of glutamatergic transmitter systems in the forebrain. Previous studies
demonstrated synaptic vesicle exchange between terminals in vitro. Using the
VGLUT1(Venus) knock-in, we show that synaptic vesicles are dynamically shared
among boutons in the cortex of mice in vivo. We provide a detailed analysis of
synaptic vesicle sharing in vitro, and show that network homeostasis leads to
dynamic scaling of synaptic VGLUT1 levels.

DOI: 10.1523/JNEUROSCI.2073-11.2011
PMID: 22031900 [Indexed for MEDLINE]

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