Identification of differentially expressed genes in the developing mouse inferior colliculus.
Developmental Brain Research. 2005-09-01; 159(1): 29-35
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1. Brain Res Dev Brain Res. 2005 Sep 8;159(1):29-35.
Identification of differentially expressed genes in the developing mouse inferior
Decourt B(1), Bouleau Y, Dulon D, Hafidi A.
(1)EA3665, Laboratoire de Biologie Cellulaire et Moleculaire de l’Audition,
Universite Victor Segalen Bordeaux 2, Hopital Pellegrin, Batiment PQR 3, 33076
Although injured neurons of inferior colliculus (IC) display a robust axonal
outgrowth through a lesion site at postnatal day six (P6) in vitro, and are
capable to re-innervate their target cells, injured neurons from P10 IC are
unable to regenerate their axons across the lesion site. This axonal regenerative
failure has been attributed to an increase of expression of inhibitory molecules
in endogenous tissue, during development. As a first step to identify such
inhibitory molecules, the present study reports the isolation of molecules
differentially expressed in the IC during development. A two-directional (forward
and backward) suppression subtractive hybridization (SSH) was performed on IC
tissue between P6 and P10 stages. One hundred cDNAs from P6 (P6-P10) and 200
cDNAs from P10 (P10-P6)-subtracted libraries were randomly sequenced. A dot-blot
screening of sequenced cDNAs revealed the differential expression for the
majority of these cDNAs at their respective developmental stage. Then, the
analysis of sequenced clones showed that P6 library was highly enriched in
molecules expressed early in the development, such as GAP43 or vimentin proteins.
By contrast, the P10 library contained mostly molecules expressed at later stages
of development in the central nervous system, such as myelin-related proteins.
Our results show that SSH is a suitable method for identifying differentially
expressed genes in the developing IC. In addition, these results provide a
foundation for further studies dealing with molecules involved in the IC
development before and at the onset of hearing, some of which being probably
involved in the axonal outgrowth mechanism.
PMID: 16095723 [Indexed for MEDLINE]