Gas chromatographic separation and mass spectrometric identification of mixtures of oxyphytosterol and oxycholesterol derivatives: Application to a phytosterol-enriched food
Journal of Chromatography A. 2004-06-01; 1040(2): 239-250
DOI: 10.1016/j.chroma.2004.04.008
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1. J Chromatogr A. 2004 Jun 25;1040(2):239-50.
Gas chromatographic separation and mass spectrometric identification of mixtures
of oxyphytosterol and oxycholesterol derivatives application to a
phytosterol-enriched food.
Grandgirard A(1), Martine L, Joffre C, Juaneda P, Berdeaux O.
Author information:
(1)Unité de Nutrition Lipidique, INRA, 17 Rue Sully, B.P. 86510, 21065 Dijon
Cedex, France.
Pure individual phytosterols were prepared using reversed-phase HPLC in order to
obtain the oxidized compounds of sitosterol, campesterol, stigmasterol and
brassicasterol. 7-Hydroxy-, 7-keto-, 5,6-epoxy-, 4beta-hydroxy-,
4-ene-6-hydroxy-, 6-keto- and 5alpha,6beta-dihydroxyphytosterols were obtained as
well as analogous compounds of cholesterol. The gas chromatographic properties as
well as the electronic impact mass spectra of these compounds (as trimethylsilyl
ether derivatives) were studied. These data were used to identify oxyphytosterols
in a spread enriched in phytosterols: the oxyphytosterols represented no more
than 68 microg/g of spread (about 0.08% of phytosterols were oxidised).
DOI: 10.1016/j.chroma.2004.04.008
PMID: 15230531 [Indexed for MEDLINE]