Functional Analysis of Mitochondrial CB1 Cannabinoid Receptors (mtCB1) in the Brain.
Methods in Enzymology. 2017-01-01; : 143-174
DOI: 10.1016/bs.mie.2017.06.023
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1. Methods Enzymol. 2017;593:143-174. doi: 10.1016/bs.mie.2017.06.023. Epub 2017 Jul
14.
Functional Analysis of Mitochondrial CB1 Cannabinoid Receptors (mtCB1) in the
Brain.
Melser S(1), Pagano Zottola AC(1), Serrat R(1), Puente N(2), Grandes P(3),
Marsicano G(4), Hebert-Chatelain E(5).
Author information:
(1)INSERM U1215, NeuroCentre Magendie, Team “Endocannabinoids and
Neuroadaptation”, Bordeaux, France; Université de Bordeaux, Bordeaux, France.
(2)Faculty of Medicine and Nursing, University of the Basque Country UPV/EHU,
Leioa, Spain; Achucarro Basque Center for Neuroscience, Science Park of the
UPV/EHU, Leioa, Spain.
(3)Faculty of Medicine and Nursing, University of the Basque Country UPV/EHU,
Leioa, Spain; Achucarro Basque Center for Neuroscience, Science Park of the
UPV/EHU, Leioa, Spain; University of Victoria, Victoria, BC, Canada.
(4)INSERM U1215, NeuroCentre Magendie, Team “Endocannabinoids and
Neuroadaptation”, Bordeaux, France; Université de Bordeaux, Bordeaux, France.
Electronic address: .
(5)Université de Moncton, Moncton, NB, Canada. Electronic address:
.
Recent evidence indicates that, besides its canonical localization at cell plasma
membranes, the type-1 cannabinoid receptor, CB1 is functionally present at brain
and muscle mitochondrial membranes (mtCB1). Through mtCB1 receptors, cannabinoids
can directly regulate intramitochondrial signaling and respiration. This new and
surprising discovery paves the way to new potential fields of research, dealing
with the direct impact of G protein-coupled receptors on bioenergetic processes
and its functional implications. In this chapter, we summarize some key
experimental approaches established in our laboratories to identify anatomical,
biochemical, and functional features of mtCB1 receptors in the brain. In
particular, we describe the procedures to obtain reliable and controlled
detection of mtCB1 receptors by immunogold electromicroscopy and by
immunoblotting methods. Then, we address the study of direct cannabinoid effects
on the electron transport system and oxidative phosphorylation. Finally, we
present a functional example of the impact of mtCB1 receptors on mitochondrial
mobility in cultured neurons. Considering the youth of the field, these
methodological approaches will very likely be improved and refined in the future,
but this chapter aims at presenting the methods that are currently used and, in
particular, at underlining the need of rigorous controls to obtain reliable
results. We hope that this chapter might help scientists becoming interested in
this new and exciting field of research.
© 2017 Elsevier Inc. All rights reserved.
DOI: 10.1016/bs.mie.2017.06.023
PMID: 28750801 [Indexed for MEDLINE]