Fluorogenic probes for monitoring peptide binding to class II MHC proteins in living cells

Prasanna Venkatraman, Tina T Nguyen, Matthieu Sainlos, Osman Bilsel, Sriram Chitta, Barbara Imperiali, Lawrence J Stern
Nat Chem Biol. 2007-03-11; 3(4): 222-228
DOI: 10.1038/nchembio868

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1. Nat Chem Biol. 2007 Apr;3(4):222-8. Epub 2007 Mar 11.

Fluorogenic probes for monitoring peptide binding to class II MHC proteins in
living cells.

Venkatraman P(1), Nguyen TT, Sainlos M, Bilsel O, Chitta S, Imperiali B, Stern

Author information:
(1)Department of Pathology, University of Massachusetts Medical School, 55 Lake
Ave. North, Worcester, Massachusetts 01655, USA.

Comment in
Nat Chem Biol. 2007 Apr;3(4):201-2.

A crucial step in the immune response is the binding of antigenic peptides to
major histocompatibility complex (MHC) proteins. Class II MHC proteins present
their bound peptides to CD4(+) T cells, thereby helping to activate both the
humoral and the cellular arms of the adaptive immune response. Peptide loading
onto class II MHC proteins is regulated temporally, spatially and developmentally
in antigen-presenting cells. To help visualize these processes, we have developed
a series of novel fluorogenic probes that incorporate the environment-sensitive
amino acid analogs 6-N,N-dimethylamino-2-3-naphthalimidoalanine and
4-N,N-dimethylaminophthalimidoalanine. Upon binding to class II MHC proteins
these fluorophores show large changes in emission spectra, quantum yield and
fluorescence lifetime. Peptides incorporating these fluorophores bind
specifically to class II MHC proteins on antigen-presenting cells and can be used
to follow peptide binding in vivo. Using these probes we have tracked a
developmentally regulated cell-surface peptide-binding activity in primary human
monocyte-derived dendritic cells.

DOI: 10.1038/nchembio868
PMCID: PMC3444530
PMID: 17351628 [Indexed for MEDLINE]

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