Extrahypophyseal expression of gonadotropin subunits in pejerrey Odontesthes bonariensis and effects of high water temperatures on their expression
General and Comparative Endocrinology. 2012-01-01; 175(2): 329-336
Read on PubMed
1. Gen Comp Endocrinol. 2012 Jan 15;175(2):329-36. doi: 10.1016/j.ygcen.2011.11.036.
Epub 2011 Nov 29.
Extrahypophyseal expression of gonadotropin subunits in pejerrey Odontesthes
bonariensis and effects of high water temperatures on their expression.
Elisio M(1), Soria FN, Fernandino JI, Strüssmann CA, Somoza GM, Miranda LA.
(1)Laboratorio de Ictiofisiología y Acuicultura, Instituto de Investigaciones
Biotecnológicas-Instituto Tecnológico de Chascomús, (B7130IWA) Chascomús, Buenos
It has been traditionally accepted that the gonadotropins (GtHs), follicle
stimulating hormone (FSH) and luteinizing hormone (LH), are synthesized and
secreted only by the pituitary. However, the presence of theses hormones in
extrapituitary tissues has been demonstrated in mammals, and more recently also
in fish. In this study, we cloned the cDNAs and characterized the expression of
FSH-β, LH-β, and glycoprotein hormone α (GPH-α) subunits from brain and gonads of
male and female pejerrey Odontesthes bonariensis at different stages of gonadal
maturation. In situ hybridization revealed that, in addition to their classical
location in pituitary cells, the three GtH transcripts were also located in the
gonads. FSH-β and GPH-α subunits were found in the cytoplasm of oogonia,
previtellogenic and vitellogenic oocytes in ovaries. LH-β expression was detected
in previtellogenic and vitellogenic oocytes but not in oogonia. In males, the
three subunits were expressed in spermatogonia and to a lesser extent in
spermatocytes. Exposure of fish to high water temperatures that impair pejerrey
reproduction also induced a decrease of extrahypophyseal expression of GtH
Copyright © 2011 Elsevier Inc. All rights reserved.
PMID: 22154644 [Indexed for MEDLINE]