Epithelial function in the Drosophila Malpighian Tubule: An in vivo renal model
Methods in Molecular Biology. 2019-01-01; : 203-221
DOI: 10.1007/978-1-4939-9021-4_17
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1. Methods Mol Biol. 2019;1926:203-221. doi: 10.1007/978-1-4939-9021-4_17.
Epithelial Function in the Drosophila Malpighian Tubule: An In Vivo Renal Model.
Davies SA(1), Cabrero P(2), Marley R(2), Corrales GM(2), Ghimire S(2), Dornan
AJ(2), Dow JAT(3).
Author information:
(1)Institute of Molecular, Cell and Systems Biology, College of Medical,
Veterinary and Life Sciences, University of Glasgow, Glasgow, Scotland, UK.
.
(2)Institute of Molecular, Cell and Systems Biology, College of Medical,
Veterinary and Life Sciences, University of Glasgow, Glasgow, Scotland, UK.
(3)Institute of Molecular, Cell and Systems Biology, College of Medical,
Veterinary and Life Sciences, University of Glasgow, Glasgow, Scotland, UK.
.
The insect renal (Malpighian) tubule has long been a model system for the study
of fluid secretion and its neurohormonal control, as well as studies on ion
transport mechanisms. To extend these studies beyond the boundaries of classical
physiology, a molecular genetic approach together with the ‘omics technologies is
required. To achieve this in any vertebrate transporting epithelium remains a
daunting task, as the genetic tools available are still relatively
unsophisticated. Drosophila melanogaster, however, is an outstanding model
organism for molecular genetics. Here we describe a technique for fluid secretion
assays in the D. melanogaster equivalent of the kidney nephron. The development
of this first physiological assay for a Drosophila epithelium, allowing combined
approaches of integrative physiology and functional genomics, has now provided
biologists with an entirely new model system, the Drosophila Malpighian tubule,
which is utilized in multiple fields as diverse as kidney disease research and
development of new modes of pest insect control.
DOI: 10.1007/978-1-4939-9021-4_17
PMID: 30742274 [Indexed for MEDLINE]