Effects of the endocrine disruptors atrazine and PCB 153 on the protein expression of MCF-7 human cells

Jean-Paul Lasserre, Fred Fack, Dominique Revets, Sébastien Planchon, Jenny Renaut, Lucien Hoffmann, Arno C. Gutleb, Claude P. Muller, Torsten Bohn
J. Proteome Res.. 2009-10-27; 8(12): 5485-5496
DOI: 10.1021/pr900480f

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1. J Proteome Res. 2009 Dec;8(12):5485-96. doi: 10.1021/pr900480f.

Effects of the endocrine disruptors atrazine and PCB 153 on the protein
expression of MCF-7 human cells.

Lasserre JP(1), Fack F, Revets D, Planchon S, Renaut J, Hoffmann L, Gutleb AC,
Muller CP, Bohn T.

Author information:
(1)Department Environment and Agro-Biotechnologies, Centre de Recherche Public –
Gabriel Lippmann, L-4422 Belvaux, Luxembourg.

Comment in
J Proteome Res. 2009 Dec;8(12):5411.

Polychlorinated biphenyls (PCBs) and a number of pesticides can act as endocrine
disrupting compounds (EDCs). These molecules exhibit hormonal activity in vivo,
and can therefore interact and perturb normal physiological functions. Many of
these compounds are persistent in the environment, and their bioaccumulation may
constitute a significant threat for human health. Physiological abnormalities
following exposure to these xenobiotic compounds go along with alterations at the
protein level of individual cells. In this study, MCF-7 cells were exposed to
environmentally relevant concentrations of atrazine, PCB153 (100 ppb,
respectively), 17-beta estradiol (positive control, 10 nM) and a negative control
(solvent) for t = 24 h (n = 3 replicates/exposure group). After trizol extraction
and protein solubilization, protein expression levels were studied by 2D-DIGE.
Proteins differentially expressed were excised, trypsin-digested, and identified
by MALDI-ToF-ToF, followed by NCBInr database search. 2D-DIGE experiments
demonstrated that 49 spots corresponding to 29 proteins were significantly
differentially expressed in MCF-7 cells (>1.5-fold, P < 0.05, Student's paired t
test). These proteins belonged to various cellular compartments (nucleus,
cytosol, membrane), and varied in function; 88% of proteins were down-regulated
during atrazine exposure, whereas 75% of proteins were up-regulated by PCB153.
Affected proteins included those regulating oxidative stress such as superoxide
dismutase and structural proteins such as actin or tropomyosin, which may explain
morphological changes of cells already observed under the microscope. This study
highlights the susceptibility of human cells to compounds with endocrine
disrupting properties.

DOI: 10.1021/pr900480f
PMID: 19778091 [Indexed for MEDLINE]

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