Effects of spaced learning in the water maze on development of dentate granule cells generated in adult mice.
Hippocampus. 2015-04-02; 25(11): 1314-1326
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1. Hippocampus. 2015 Nov;25(11):1314-26. doi: 10.1002/hipo.22438. Epub 2015 Apr 2.
Effects of spaced learning in the water maze on development of dentate granule
cells generated in adult mice.
Trinchero MF(1), Koehl M(2)(3), Bechakra M(2)(3), Delage P(2)(3), Charrier
V(2)(3), Grosjean N(2)(3), Ladeveze E(2)(3), Schinder AF(1), Abrous DN(2)(3).
(1)Laboratory of Neuronal Plasticity, Leloir Institute, Consejo Nacional De
Investigaciones Científicas Y Técnicas, Buenos Aires, Argentina.
(2)Inserm U862, Bordeaux, France.
(3)Université De Bordeaux, Bordeaux, France.
New dentate granule cells (GCs) are generated in the hippocampus throughout life.
These adult-born neurons are required for spatial learning in the Morris water
maze (MWM). In rats, spatial learning shapes the network by regulating their
number and dendritic development. Here, we explored whether such modulatory
effects exist in mice. New GCs were tagged using thymidine analogs or a
GFP-expressing retrovirus. Animals were exposed to a reference memory protocol
for 10-14 days (spaced training) at different times after newborn cells labeling.
Cell proliferation, cell survival, cell death, neuronal phenotype, and dendritic
and spine development were examined using immunohistochemistry. Surprisingly,
spatial learning did not modify any of the parameters under scrutiny including
cell number and dendritic morphology. These results suggest that although new GCs
are required in mice for spatial learning in the MWM, they are, at least for the
developmental intervals analyzed here, refractory to behavioral stimuli generated
in the course of learning in the MWM.
© 2015 Wiley Periodicals, Inc.
PMID: 25740272 [Indexed for MEDLINE]