Early morphofunctional plasticity of microglia in response to acute lipopolysaccharide

C. Madore, C. Joffre, J.C. Delpech, V. De Smedt-Peyrusse, A. Aubert, L. Coste, S. Layé, A. Nadjar
Brain, Behavior, and Immunity. 2013-11-01; 34: 151-158
DOI: 10.1016/j.bbi.2013.08.008

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1. Brain Behav Immun. 2013 Nov;34:151-8. doi: 10.1016/j.bbi.2013.08.008. Epub 2013
Aug 29.

Early morphofunctional plasticity of microglia in response to acute

Madore C(1), Joffre C, Delpech JC, De Smedt-Peyrusse V, Aubert A, Coste L, Layé
S, Nadjar A.

Author information:
(1)INRA, Nutrition and Integrative Neurobiology, UMR 1286, F-33000 Bordeaux,
France; Univ. Bordeaux, Nutrition and Integrative Neurobiology, UMR 1286, F-33000
Bordeaux, France.

Within the central nervous system (CNS) the traditional role of microglia has
been in brain infection and disease, phagocytosing debris and secreting factors
to modify disease progression. This led to the concept of “resting” versus
“activated” microglia. However, this is misleading because multiple phenotypic
and morphological stages of microglia can influence neuronal structure and
function in any condition and recent evidence extends their role to healthy brain
homeostasis. The present work was thus aimed at reappraising the concept of
morphofunctional activity of microglia in a context of peripheral acute immune
challenge, where microglial activity is known to be modified, using the new
state-of-the-art techniques available. To do so, mice were injected peripherally
with lipopolysaccharide, a potent inducer of cerebral inflammation, and we
assessed early cytokines production, phenotype, motility and morphology of
microglial cells. Our results showed that LPS induced a widespread inflammatory
response both peripherally and centrally, as revealed by the quantification of
cytokines levels. We also found an alteration of microglial motility that was
confirmed by in vivo studies showing an overall reduction of microglial processes
length in the hippocampus of LPS-treated animals. Finally, analysis of various
surface receptors expression revealed that LPS did not significantly impact
microglial phenotype 2h after the injection but rather induced an increase of
CD11b(+)/CD45(high) cells. These latter may be at the vasculature, at the CNS
vicinity, or may have invaded the CNS.

Copyright © 2013 Elsevier Inc. All rights reserved.

DOI: 10.1016/j.bbi.2013.08.008
PMID: 23994463 [Indexed for MEDLINE]

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