Distribution of the lipolysis stimulated receptor in adult and embryonic murine tissues and lethality of LSR-/- Embryos at 12.5 to 14.5 days of gestation

Samir Mesli, Sandrine Javorschi, Annie M. Bérard, Marc Landry, Helen Priddle, David Kivlichan, Andrew J. H. Smith, Frances T. Yen, Bernard E. Bihain, Michel Darmon
European Journal of Biochemistry. 2004-07-06; 271(15): 3103-3114
DOI: 10.1111/j.1432-1033.2004.04223.x

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1. Eur J Biochem. 2004 Aug;271(15):3103-14.

Distribution of the lipolysis stimulated receptor in adult and embryonic murine
tissues and lethality of LSR-/- embryos at 12.5 to 14.5 days of gestation.

Mesli S(1), Javorschi S, Bérard AM, Landry M, Priddle H, Kivlichan D, Smith AJ,
Yen FT, Bihain BE, Darmon M.

Author information:
(1)Laboratoire de Biochimie et de Biologie Moléculaire, Université Victor Ségalen
Bordeaux 2, France.

The lipolysis stimulated receptor (LSR) recognizes apolipoprotein B/E-containing
lipoproteins in the presence of free fatty acids, and is thought to be involved
in the clearance of triglyceride-rich lipoproteins (TRL). The distribution of LSR
in mice was studied by Northern blots, quantitative PCR and immunofluorescence.
In the adult, LSR mRNA was detectable in all tissues tested except muscle and
heart, and was abundant in liver, lung, intestine, kidney, ovaries and testes.
During embryogenesis, LSR mRNA was detectable at 7.5 days post-coitum (E7) and
increased up to E17 in parallel to prothrombin, a liver marker. In adult liver,
immunofluorescence experiments showed a staining at the periphery of hepatocytes
as well as in fetal liver at E12 and E15. These results are in agreement with the
assumption that LSR is a plasma membrane receptor involved in the clearance of
lipoproteins by liver, and suggest a possible role in steroidogenic organs, lung,
intestine and kidney). To explore the role of LSR in vivo, the LSR gene was
inactivated in 129/Ola ES cells by removing a gene segment containing exons 2-5,
and 129/Ola-C57BL/6 mice bearing the deletion were produced. Although
heterozygotes appeared normal, LSR homozygotes were not viable, with the
exception of three males, while the total progeny of genotyped wild-type and
heterozygote pups was 345. Mortality of the homozygote embryos was observed
between days 12.5 and 15.5 of gestation, a time at which their liver was much
smaller than that of their littermates, indicating that the expression of LSR is
critical for liver and embryonic development.

DOI: 10.1111/j.1432-1033.2004.04223.x
PMID: 15265030 [Indexed for MEDLINE]

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