Differential expression of nm23 genes in adult mouse dorsal root ganglia

Perrine Barraud, Lilian Amrein, Eric Dobremez, Sandrine Dabernat, Karine Masse, Monique Larou, Jean-Yves Daniel, Marc Landry
J. Comp. Neurol.. 2002-02-20; 444(4): 306-323
DOI: 10.1002/cne.10150

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1. J Comp Neurol. 2002 Mar 18;444(4):306-23.

Differential expression of nm23 genes in adult mouse dorsal root ganglia.

Barraud P(1), Amrein L, Dobremez E, Dabernat S, Masse K, Larou M, Daniel JY,
Landry M.

Author information:
(1)EA DRED 483, Laboratoire de Biologie de la Différenciation et du
Développement, Université Victor Ségalen, 33 076 Bordeaux Cedex, France.

Nm23 has been identified as a gene family encoding different isoforms of
nucleoside diphosphate kinase (NDPK). This protein is a key enzyme in nucleotide
metabolism and has been shown to play important roles in various cellular
functions. In the present study, we have investigated the expression of three
isotypes in mouse dorsal root ganglia. In situ hybridization and reverse
transcriptase-polymerase chain reaction analysis demonstrated high levels of
nm23-M1, -M2, and -M3 mRNA expression in peripheral nervous tissue. Moreover, in
situ hybridization also displayed a specific nuclear localization for nm23-M2
mRNA. Immunohistochemistry with light and electron microscopy on isoform-specific
antibodies revealed a differential subcellular distribution of NDPK isoforms.
Isoform A was mainly cytosolic, showing only partial association with organelles.
In contrast, isoform B was also found in the nucleus, which is in agreement with
its proposed role as a transcription factor. The results also indicate a
preferential association of isoform C with endoplasmic reticulum and plasma
membranes in neuronal cells. Furthermore, isoform C appeared to combine with
other NDPK isoforms as demonstrated by double-labeling evidence by electron
microscopy and might be responsible for binding NDPK oligomers to membranes.
Thus, isoform C may be considered as a protein of importance for maintaining
intracellular pools of GTP in the vicinity of membranes and, hence, for
transmembrane signaling. The results indicate a high expression of NDPK isoforms,
not only in the central but also in the peripheral nervous system. Their
different subcellular compartmentalization suggests that they have
isoform-specific roles in neuronal cell physiology.

Copyright 2002 Wiley-Liss, Inc.

DOI: 10.1002/cne.10150
PMID: 11891645 [Indexed for MEDLINE]

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