Concentration landscape generators for shear free dynamic chemical stimulation
Lab Chip. 2012-01-01; 12(7): 1340
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1. Lab Chip. 2012 Apr 7;12(7):1340-6. doi: 10.1039/c2lc20994b. Epub 2012 Feb 20.
Concentration landscape generators for shear free dynamic chemical stimulation.
Morel M(1), Galas JC, Dahan M, Studer V.
(1)Laboratoire Kastler Brossel, CNRS UMR8552, Département de Physique et Institut
de Biologie, Ecole Normale Supérieure, Université Pierre et Marie Curie – Paris
6, 46 rue d’Ulm, 75005 Paris, France.
In this paper we first introduce a novel fabrication process, which allows for
easy integration of thin track-etched nanoporous membranes, within 2D or 3D
microchannel networks. In these networks, soluble chemical compounds can diffuse
out of the channels through well-defined and spatially organized microfabricated
porous openings. Interestingly, multiple micron-scale porous areas can be
integrated in the same device and each of these areas can be connected to a
different microfluidic channel and reservoir. We then present and characterize
several membrane-based microdevices and their use for the generation of stable
diffusible concentration gradients and complex dynamic chemical landscapes under
shear free conditions. We also demonstrate how a simple flow-focusing geometry
can be used to generate “on-demand” concentration profiles. In turn, these
devices should provide an ideal experimental framework for high throughput
cell-based assays: long term high-resolution video microscopy experiments can be
performed, under multiple spatially and temporally controlled chemical
conditions, with simple protocols and in a cell-friendly environment.
This journal is © The Royal Society of Chemistry 2012
PMID: 22344388 [Indexed for MEDLINE]