Cerebral Cortex Electroporation to Study Projection Neuron Migration.
Current Protocols in Neuroscience. 2016-10-01; 77(1): 2.26.1-2.26.18
DOI: 10.1002/cpns.13
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1. Curr Protoc Neurosci. 2016 Oct 3;77:2.26.1-2.26.18. doi: 10.1002/cpns.13.
Cerebral Cortex Electroporation to Study Projection Neuron Migration.
Pacary E(1)(2), Guillemot F(3).
Author information:
(1)INSERM U1215, Neurocentre Magendie, Bordeaux, France.
(2)Université de Bordeaux, Bordeaux, France.
(3)The Francis Crick Institute, Mill Hill Laboratory, London, United Kingdom.
Brain electroporation is a rapid and powerful approach to study neuronal
development. In particular, this technique has become a method of choice for
studying the process of radial migration of projection neurons in the embryonic
cerebral cortex. This method has considerably helped to describe in detail the
different steps of radial migration and to characterize the molecular mechanisms
controlling this process. Delineating the complexities of neuronal migration is
critical to our understanding not only of normal cerebral cortex formation but
also of neurodevelopmental disorders resulting from neuronal migration defects.
Here, we describe in detail the protocols to perform in utero or ex vivo
electroporation of progenitor cells in the ventricular zone of the cerebral
cortex with the aim of studying the process of radial migration of projection
neurons during embryonic development. © 2016 by John Wiley & Sons, Inc.
Copyright © 2016 John Wiley & Sons, Inc.
DOI: 10.1002/cpns.13
PMID: 27696363 [Indexed for MEDLINE]