Brain extracellular matrix affects AMPA receptor lateral mobility and short-term synaptic plasticity.
Nat Neurosci. 2009-05-31; 12(7): 897-904
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1. Nat Neurosci. 2009 Jul;12(7):897-904. doi: 10.1038/nn.2338. Epub 2009 May 31.
Brain extracellular matrix affects AMPA receptor lateral mobility and short-term
Frischknecht R(1), Heine M, Perrais D, Seidenbecher CI, Choquet D, Gundelfinger
(1)Leibniz Institute for Neurobiology, Magdeburg, Germany.
Many synapses in the mature CNS are wrapped by a dense extracellular matrix
(ECM). Using single-particle tracking and fluorescence recovery after
photobleaching, we found that this net-like ECM formed surface compartments on
rat primary neurons that acted as lateral diffusion barriers for AMPA-type
glutamate receptors. Enzymatic removal of the ECM increased extrasynaptic
receptor diffusion and the exchange of synaptic AMPA receptors. Whole-cell
patch-clamp recording revealed an increased paired-pulse ratio as a functional
consequence of ECM removal. These results suggest that the surface compartments
formed by the ECM hinder lateral diffusion of AMPA receptors and may therefore
modulate short-term synaptic plasticity.
PMID: 19483686 [Indexed for MEDLINE]