A Spatiotemporal Sequence of Sensitization to Slits and Semaphorins Orchestrates Commissural Axon Navigation.
Cell Reports. 2019-10-01; 29(2): 347-362.e5
DOI: 10.1016/j.celrep.2019.08.098
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1. Cell Rep. 2019 Oct 8;29(2):347-362.e5. doi: 10.1016/j.celrep.2019.08.098.
A Spatiotemporal Sequence of Sensitization to Slits and Semaphorins Orchestrates
Commissural Axon Navigation.
Pignata A(1), Ducuing H(1), Boubakar L(1), Gardette T(1), Kindbeiter K(1), Bozon
M(1), Tauszig-Delamasure S(1), Falk J(1), Thoumine O(2), Castellani V(3).
Author information:
(1)University of Lyon, University of Lyon 1 Claude Bernard Lyon1, NeuroMyoGene
Institute, CNRS UMR5310, INSERM U1217, 8 Avenue Rockefeller, 69008 Lyon, France.
(2)Interdisciplinary Institute for Neuroscience, UMR CNRS 5297, University of
Bordeaux 146 rue Léo Saignat, 33000 Bordeaux, France.
(3)University of Lyon, University of Lyon 1 Claude Bernard Lyon1, NeuroMyoGene
Institute, CNRS UMR5310, INSERM U1217, 8 Avenue Rockefeller, 69008 Lyon, France.
Electronic address: .
Accurate perception of guidance cues is crucial for cell and axon migration.
During initial navigation in the spinal cord, commissural axons are kept
insensitive to midline repellents. Upon midline crossing in the floor plate, they
switch on responsiveness to Slit and Semaphorin repulsive signals and are thus
propelled away and prevented from crossing back. Whether and how the different
midline repellents control specific aspects of this navigation remain to be
elucidated. We set up a paradigm for live-imaging and super-resolution analysis
of PlexinA1, Neuropilin-2, and Robo1/2 receptor dynamics during commissural
growth cone navigation in chick and mouse embryos. We uncovered a remarkable
program of sensitization to midline cues achieved by unique spatiotemporal
sequences of receptor allocation at the growth-cone surface that orchestrates
receptor-specific growth-cone behavior changes. This reveals post-translational
mechanisms whereby coincident guidance signals are temporally resolved to allow
the generation of specific guidance responses.
Copyright © 2019 The Authors. Published by Elsevier Inc. All rights reserved.
DOI: 10.1016/j.celrep.2019.08.098
PMID: 31597096 [Indexed for MEDLINE]