Whole-cell voltage clamp on skeletal muscle fibers with the silicone-clamp technique
Methods in Molecular Biology. 2014-01-01; : 159-170
DOI: 10.1007/978-1-4939-1096-0_9
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Lefebvre R(1), Pouvreau S, Collet C, Allard B, Jacquemond V.
Author information:
(1)Centre de Génétique et de Physiologie Moléculaire et Cellulaire, UMR CNRS
5534, Université Claude Bernard – Lyon 1, Bât. Raphael Dubois, 43 boulevard du
11 novembre 1918, Villeurbanne Cedex, 69622, France.
Control of membrane voltage and membrane current measurements are of critical
importance for the study of numerous aspects of skeletal muscle physiology and
pathophysiology. The silicone-clamp technique makes use of a conventional
patch-clamp apparatus to achieve whole-cell voltage clamp of a restricted
portion of a fully differentiated adult skeletal muscle fiber. The major part of
an isolated muscle fiber is insulated from the extracellular medium with
silicone grease and the tip of a single microelectrode connected to the
amplifier is then inserted within the fiber through the silicone layer. The
method is extremely easy to implement. It represents an alternative to the
traditional vaseline-gap isolation and two or three microelectrodes
voltage-clamp techniques. The present chapter reviews the benefits of the
silicone-clamp technique and provides updated detailed insights into its
practical implementation.
DOI: 10.1007/978-1-4939-1096-0_9
PMID: 25023307 [Indexed for MEDLINE]