Usefulness of autoantigens depletion to detect autoantibody signatures by multiple affinity protein profiling
J. Sep. Sci.. 2007-02-01; 30(3): 352-358
DOI: 10.1002/jssc.200600324
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1. J Sep Sci. 2007 Feb;30(3):352-8.
Usefulness of autoantigens depletion to detect autoantibody signatures by
multiple affinity protein profiling.
Hardouin J(1), Lasserre JP, Canelle L, Duchateau M, Vlieghe C,
Choquet-Kastylevsky G, Joubert-Caron R, Caron M.
Author information:
(1)Laboratory of Protein Biochemistry and Proteomics, UMR CNRS 7033 (BioMoCeTi),
UFR SMBH, University Paris 13, Bobigny cedex, France.
Patients with cancer produce specific autoantibodies against protein antigens
present in limited amount among a large background of immunoglobulins (Igs),
nonrelevant as biomarkers, including natural antibodies. Multiple affinity
protein profiling (MAPPing) that combines 2-D immunoaffinity chromatography,
enzymatic digestion of the isolated proteins, and identification by MS/MS, may
facilitate the identification of these so far unknown patient antibodies. The
first immunoaffinity chromatography is crucial, as it is used for selectively
removing proteins (autoantigens) recognized by natural antibodies. Application of
this depletion step to colon cancer cell proteins is specifically described along
with the identification of the natural autoantigens, as well as the coupling of
this depletion step with the next steps. By enabling to separate antibody-binding
proteins recognized by either natural autoantibodies or patient-specific
antibodies this approach may contribute significantly towards the definition of
autoantibody signatures.
DOI: 10.1002/jssc.200600324
PMID: 17396593 [Indexed for MEDLINE]