Two-Color STED Imaging of Synapses in Living Brain Slices
Nanoimaging. 2012-09-18; : 65-80
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1. Methods Mol Biol. 2013;950:65-80. doi: 10.1007/978-1-62703-137-0_5.
Two-color STED imaging of synapses in living brain slices.
Tønnesen J(1), Nägerl UV.
(1)Interdisciplinary Institute for Neuroscience (IINS), Université de Bordeaux,
STED microscopy is a novel fluorescence microscopy technique that breaks the
classic diffraction barrier of optical microscopy. It offers the chance to
investigate dynamic processes inside living cells with a spatial resolution well
below 100 nm, possibly even down to a few nanometers, essentially without
forgoing the benefits of conventional light microscopy, such as labeling
specificity, sensitivity, and contrast. STED microscopy has already been
exploited for several important neurobiological experiments. Given the tremendous
potential as a transforming technology, it is important to understand how it
works, and what its scope and limitations are. Here, we present a primer on STED
microscopy, its basic principles and practical implementation, presenting a
how-to guide on building and operating a STED microscope.
PMID: 23086870 [Indexed for MEDLINE]