Transcription factor EB overexpression prevents neurodegeneration in experimental synucleinopathies

Marie-Laure Arotcarena, Mathieu Bourdenx, Nathalie Dutheil, Marie-Laure Thiolat, Evelyne Doudnikoff, Sandra Dovero, Andrea Ballabio, Pierre-Olivier Fernagut, Wassilios G. Meissner, Erwan Bezard, Benjamin Dehay
JCI Insight. 2019-08-22; 4(16):
DOI: 10.1172/jci.insight.129719

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Arotcarena ML(1)(2), Bourdenx M(1)(2), Dutheil N(1)(2), Thiolat ML(1)(2), Doudnikoff E(1)(2), Dovero S(1)(2), Ballabio A(3)(4)(5), Fernagut PO(6), Meissner WG(1)(2)(7), Bezard E(1)(2), Dehay B(1)(2).

Author information:
(1)Université de Bordeaux, Institut des Maladies Neurodégénératives, UMR 5293, F-33000 Bordeaux, France.
(2)CNRS, Institut des Maladies Neurodégénératives, UMR 5293, F-33000 Bordeaux, France.
(3)Telethon Institute of Genetics and Medicine (TIGEM), Pozzuoli (Naples), Italy.
(4)Department of Translational Medicine, Federico II University, Naples, Italy.
(5)Department of Molecular and Human Genetics, Ian and Dan Duncan Neurological Research Institute, Baylor College of Medicine, Houston, Texas, USA.
(6)Laboratoire de Neurosciences Expérimentales et Cliniques, INSERM U-1084, Université de Poitiers, Poitiers, France.
(7)Service de Neurologie, Centre Hospitalier Universitaire de Bordeaux, Bordeaux, France.

Abstract

The synucleinopathies Parkinson’s disease (PD) and Multiple system atrophy (MSA) – characterized by α-synuclein intracytoplasmic inclusions into, respectively, neurons and oligodendrocytes – are associated with impairment of the autophagy-lysosomal pathways (ALP). Increased expression of the master regulator of ALP, transcription factor EB (TFEB), is hypothesized to promote the clearance of WT α-synuclein and survival of dopaminergic neurons. Here, we explore the efficacy of targeted TFEB overexpression either in neurons or oligodendrocytes to reduce the pathological burden of α-synuclein in a PD rat model and a MSA mouse model. While TFEB neuronal expression was sufficient to prevent  neurodegeneration in the PD model, we show that only TFEB oligodendroglial overexpression leads to neuroprotective effects in the MSA model. These beneficial effects were associated with a decreased accumulation of α-synuclein into oligodendrocytes through recovery of the ALP machinery. Our study demonstrates that the cell type where α-synuclein aggregates dictates the target of TFEB overexpression in order to be protective, paving the way for adapted therapies.

DOI: 10.1172/jci.insight.129719
PMID: 31434803


Auteurs Bordeaux Neurocampus