The Interaction between Stargazin and PSD-95 Regulates AMPA Receptor Surface Trafficking

Cecile Bats, Laurent Groc, Daniel Choquet
Neuron. 2007-03-01; 53(5): 719-734
DOI: 10.1016/j.neuron.2007.01.030

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1. Neuron. 2007 Mar 1;53(5):719-34.

The interaction between Stargazin and PSD-95 regulates AMPA receptor surface

Bats C(1), Groc L, Choquet D.

Author information:
(1)Physiologie Cellulaire de la Synapse, UMR 5091 CNRS – Institut François
Magendie, Université Bordeaux, Bordeaux 33077, France.

Accumulation of AMPA receptors at synapses is a fundamental feature of
glutamatergic synaptic transmission. Stargazin, a member of the TARP family, is
an AMPAR auxiliary subunit allowing interaction of the receptor with scaffold
proteins of the postsynaptic density, such as PSD-95. How PSD-95 and Stargazin
regulate AMPAR number in synaptic membranes remains elusive. We show, using
single quantum dot and FRAP imaging in live hippocampal neurons, that exchange of
AMPAR by lateral diffusion between extrasynaptic and synaptic sites mostly
depends on the interaction of Stargazin with PSD-95 and not upon the GluR2 AMPAR
subunit C terminus. Disruption of interactions between Stargazin and PSD-95
strongly increases AMPAR surface diffusion, preventing AMPAR accumulation at
postsynaptic sites. Furthermore, AMPARs and Stargazin diffuse as complexes in and
out synapses. These results propose a model in which the Stargazin-PSD-95
interaction plays a key role to trap and transiently stabilize diffusing AMPARs
in the postsynaptic density.

DOI: 10.1016/j.neuron.2007.01.030
PMID: 17329211 [Indexed for MEDLINE]

Auteurs Bordeaux Neurocampus