The excitatory postsynaptic density is a size exclusion diffusion environment
Neuropharmacology. 2009-01-01; 56(1): 30-36
DOI: 10.1016/j.neuropharm.2008.07.022
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1. Neuropharmacology. 2009 Jan;56(1):30-6. doi: 10.1016/j.neuropharm.2008.07.022.
Epub 2008 Jul 23.
The excitatory postsynaptic density is a size exclusion diffusion environment.
Renner ML(1), Cognet L, Lounis B, Triller A, Choquet D.
Author information:
(1)Physiologie Cellulaire de la Synapse, CNRS (UMR 5091), Université Bordeaux,
Institut François Magendie, 146 rue Leo Saignat, 33077 Bordeaux Cedex, France.
Receptors are concentrated in the postsynaptic membrane but can enter and exit
synapses rapidly during both basal turnover and processes of synaptic plasticity.
How the exchange of receptors by lateral diffusion between synaptic and
extrasynaptic areas is regulated remains largely unknown. We investigated the
structural properties of the postsynaptic membrane that allow these movements by
addressing the diffusion behaviors of AMPA receptors (AMPARs) and different
lipids. Using single molecule tracking we found that not only AMPARs but also
lipids, which are not synaptically enriched, display confined diffusion at
synapses. Each molecule type displays a different average confinement area,
smaller molecules being confined to smaller areas. Glutamate application
increases the mobility of all molecules. The structure of the synaptic membrane
is thus probably organized as a size exclusion matrix and this controls the rate
of exchange of molecules with the extrasynaptic membrane.
DOI: 10.1016/j.neuropharm.2008.07.022
PMID: 18694768 [Indexed for MEDLINE]