Spatial localization of sources in the rat subthalamic motor region using an inverse current source density method

Kees J. van Dijk, Marcus L. F. Janssen, Daphne G. M. Zwartjes, Yasin Temel, Veerle Visser-Vandewalle, Peter H. Veltink, Abdelhamid Benazzouz, Tjitske Heida
Front. Neural Circuits. 2016-11-03; 10:
DOI: 10.3389/fncir.2016.00087

PubMed
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van Dijk KJ(1), Janssen ML(2), Zwartjes DG(1), Temel Y(3), Visser-Vandewalle V(4), Veltink PH(1), Benazzouz A(5), Heida T(1).

Author information:
(1)Biomedical Signals and Systems Group, MIRA institute for Biomedical
Engineering and Technical Medicine, University of Twente Enschede, Netherlands.
(2)Department of Neuroscience, School for Mental Health and Neuroscience,
Maastricht UniversityMaastricht, Netherlands; Department of Neurology,
Maastricht University Medical CenterMaastricht, Netherlands; University de
Bordeaux, Institut des Maladies Neurodégénératives, Centre National de la
Recherche Scientifique UMR 5293Bordeaux, France.
(3)Department of Neuroscience, School for Mental Health and Neuroscience,
Maastricht UniversityMaastricht, Netherlands; Department of Neurosurgery,
Maastricht University Medical CenterMaastricht, Netherlands.
(4)Department of Stereotactic and Functional Neurosurgery, University of Cologne
Cologne, Germany.
(5)University de Bordeaux, Institut des Maladies Neurodégénératives, Centre
National de la Recherche Scientifique UMR 5293 Bordeaux, France.

Objective: In this study we introduce the use of the current source density
(CSD) method as a way to visualize the spatial organization of evoked responses
in the rat subthalamic nucleus (STN) at fixed time stamps resulting from motor
cortex stimulation. This method offers opportunities to visualize neuronal input
and study the relation between the synaptic input and the neural output of
neural populations. Approach: Motor cortex evoked local field potentials and
unit activity were measured in the subthalamic region, with a 3D measurement
grid consisting of 320 measurement points and high spatial resolution. This
allowed us to visualize the evoked synaptic input by estimating the current
source density (CSD) from the measured local field potentials, using the inverse
CSD method. At the same time, the neuronal output of the cells within the grid
is assessed by calculating post stimulus time histograms. Main results: The CSD
method resulted in clear and distinguishable sources and sinks of the neuronal
input activity in the STN after motor cortex stimulation. We showed that the
center of the synaptic input of the STN from the motor cortex is located dorsal
to the input from globus pallidus. Significance: For the first time we have
performed CSD analysis on motor cortex stimulation evoked LFP responses in the
rat STN as a proof of principle. Our results suggest that the CSD method can be
used to gain new insights into the spatial extent of synaptic pathways in brain
structures.

 

Auteurs Bordeaux Neurocampus