Single molecule imaging unveils cellular architecture, dynamics and mechanobiology.
Current Opinion in Cell Biology. 2024-06-01; 88: 102369
DOI: 10.1016/j.ceb.2024.102369
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Chen T(1), Giannone G(2).
Author information:
(1)Interdisciplinary Institute for Neuroscience, Université Bordeaux, CNRS, UMR
5297, 33000 Bordeaux, France.
(2)Interdisciplinary Institute for Neuroscience, Université Bordeaux, CNRS, UMR
5297, 33000 Bordeaux, France. Electronic address:
.
The biomechanical regulation of the cytoskeleton and cell adhesions underlies
various essential cellular functions. Studying them requires visualizing their
nanostructure and molecular dynamics with evermore precise spatio-temporal
resolution. In this review we will focus on the recent advances in single
molecule fluorescence imaging techniques and discuss how they improve our
understanding of mechanically sensitive cellular structures such as adhesions
and the cytoskeleton. We will also discuss future directions for research,
emphasizing on the 3D nature of cellular structures and tissues, their
mechanical regulation at the molecule level, as well as how super-resolution
microscopy will enhance our knowledge on protein structure and conformational
changes in the cellular context.
Copyright © 2024 Elsevier Ltd. All rights reserved.
DOI: 10.1016/j.ceb.2024.102369
PMID: 38759257 [Indexed for MEDLINE]