Single-molecule imaging of the functional crosstalk between surface NMDA and dopamine D1 receptors.
Proceedings of the National Academy of Sciences. 2013-10-14; 110(44): 18005-18010
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1. Proc Natl Acad Sci U S A. 2013 Oct 29;110(44):18005-10. doi:
10.1073/pnas.1310145110. Epub 2013 Oct 14.
Single-molecule imaging of the functional crosstalk between surface NMDA and
dopamine D1 receptors.
Ladepeche L(1), Dupuis JP, Bouchet D, Doudnikoff E, Yang L, Campagne Y, Bézard E,
Hosy E, Groc L.
(1)Interdisciplinary Institute for Neuroscience, Unité Mixte de Recherche (UMR)
5297, Université de Bordeaux, F-33000 Bordeaux, France.
Dopamine is a powerful modulator of glutamatergic neurotransmission and NMDA
receptor-dependent synaptic plasticity. Although several intracellular cascades
participating in this functional dialogue have been identified over the last few
decades, the molecular crosstalk between surface dopamine and glutamate NMDA
receptor (NMDAR) signaling still remains poorly understood. Using a combination
of single-molecule detection imaging and electrophysiology in live hippocampal
neurons, we demonstrate here that dopamine D1 receptors (D1Rs) and NMDARs form
dynamic surface clusters in the vicinity of glutamate synapses. Strikingly, D1R
activation or D1R/NMDAR direct interaction disruption decreases the size of these
clusters, increases NMDAR synaptic content through a fast lateral redistribution
of the receptors, and favors long-term synaptic potentiation. Together, these
data demonstrate the presence of dynamic D1R/NMDAR perisynaptic reservoirs
favoring a rapid and bidirectional surface crosstalk between receptors and set
the plasma membrane as the primary stage of the dopamine-glutamate interplay.
PMID: 24127604 [Indexed for MEDLINE]