Shifts of Faecal Microbiota During Sporadic Colorectal Carcinogenesis.
Sci Rep. 2018-07-09; 8(1):
DOI: 10.1038/s41598-018-28671-9
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1. Sci Rep. 2018 Jul 9;8(1):10329. doi: 10.1038/s41598-018-28671-9.
Shifts of Faecal Microbiota During Sporadic Colorectal Carcinogenesis.
Mori G(1), Rampelli S(2), Orena BS(3), Rengucci C(4), De Maio G(4), Barbieri
G(3), Passardi A(5), Casadei Gardini A(5), Frassineti GL(5), Gaiarsa S(6)(7),
Albertini AM(3), Ranzani GN(3), Calistri D(4), Pasca MR(8).
Author information:
(1)Department of Biology and Biotechnology « Lazzaro Spallanzani », University of
Pavia, Pavia, Italy. .
(2)Unit of Microbial Ecology of Health, Department of Pharmacy and Biotechnology,
University of Bologna, Bologna, Italy.
(3)Department of Biology and Biotechnology « Lazzaro Spallanzani », University of
Pavia, Pavia, Italy.
(4)Biosciences Laboratory, Istituto Scientifico Romagnolo per lo Studio e la Cura
dei Tumori (I.R.S.T.), Meldola (FC), Italy.
(5)Department of Medical Oncology, Istituto Scientifico Romagnolo per lo Studio e
la Cura dei Tumori (IRST) IRCCS, Meldola (FC), Italy.
(6)S.C. Microbiologia e Virologia, Fondazione IRCCS Policlinico San Matteo,
Pavia, Italy.
(7)Dipartimento di Bioscienze, Università degli Studi di Milano, Milan, Italy.
(8)Department of Biology and Biotechnology « Lazzaro Spallanzani », University of
Pavia, Pavia, Italy. .
Gut microbiota has been implicated in the etiopathogenesis of colorectal cancer.
The development of colorectal cancer is a multistep process by which healthy
epithelium slowly develops into preneoplastic lesions, which in turn progress
into malignant carcinomas over time. In particular, sporadic colorectal cancers
can arise from adenomas (about 85% of cases) or serrated polyps through the
« adenoma-carcinoma » or the « serrated polyp-carcinoma » sequences, respectively. In
this study, we performed 16 S rRNA gene sequencing of bacterial DNA extracted
from faecal samples to compare the microbiota of healthy subjects and patients
with different preneoplastic and neoplastic lesions. We identified putative
microbial biomarkers associated with stage-specific progression of colorectal
cancer. In particular, bacteria belonging to the Firmicutes and Actinobacteria
phyla, as well as members of the Lachnospiraceae family, proved to be specific of
the faecal microbiota of patients with preneoplastic lesions, including adenomas
and hyperplastic polyps. On the other hand, two families of the Proteobacteria
phylum, Alcaligeneaceae and Enterobacteriaceae, with Sutterella and
Escherichia/Shigella being the most representative genera, appeared to be
associated with malignancy. These findings, once confirmed on larger cohorts of
patients, can represent an important step towards the development of more
effective diagnostic strategies.
DOI: 10.1038/s41598-018-28671-9
PMCID: PMC6037773
PMID: 29985435 [Indexed for MEDLINE]