Self-propelling vesicles define glycolysis as the minimal energy machinery for neuronal transport.

María-Victoria Hinckelmann, Amandine Virlogeux, Christian Niehage, Christel Poujol, Daniel Choquet, Bernard Hoflack, Diana Zala, Frédéric Saudou
Nat Comms. 2016-10-24; 7: 13233
DOI: 10.1038/ncomms13233

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1. Nat Commun. 2016 Oct 24;7:13233. doi: 10.1038/ncomms13233.

Self-propelling vesicles define glycolysis as the minimal energy machinery for
neuronal transport.

Hinckelmann MV(1)(2)(3)(4), Virlogeux A(1)(2)(3)(4)(5)(6), Niehage C(7), Poujol
C(8)(9), Choquet D(8)(9), Hoflack B(7), Zala D(1)(2)(3), Saudou
F(1)(2)(3)(5)(6)(10).

Author information:
(1)Institut Curie, F-91405 Orsay, France.
(2)CNRS, UMR3306, F-91405 Orsay, France.
(3)Inserm, U1005, F-91405 Orsay, France.
(4)Faculté de Médecine, Univ. Paris Sud11, F-94276 Le Kremlin-Bicêtre, France.
(5)Grenoble Institut des Neurosciences, GIN, Univ. Grenoble Alpes, F-38000
Grenoble, France.
(6)Inserm, U1216, F-38000 Grenoble, France.
(7)Biotechnology Center, Technische Universität Dresden, D-01307 Dresden,
Germany.
(8)CNRS, UMR 5297, F-33000 Bordeaux, France.
(9)Interdisciplinary Institute for Neuroscience, IINS, Univ. Bordeaux, F-33077
Bordeaux, France.
(10)CHU Grenoble Alpes, F-38000 Grenoble, France.

The glycolytic enzyme glyceraldehyde-3-phosphate dehydrogenase (GAPDH)
facilitates fast axonal transport in neurons. However, given that GAPDH does not
produce ATP, it is unclear whether glycolysis per se is sufficient to propel
vesicles. Although many proteins regulating transport have been identified, the
molecular composition of transported vesicles in neurons has yet to be fully
elucidated. Here we selectively enrich motile vesicles and perform quantitative
proteomic analysis. In addition to the expected molecular motors and vesicular
proteins, we find an enrichment of all the glycolytic enzymes. Using biochemical
approaches and super-resolution microscopy, we observe that most glycolytic
enzymes are selectively associated with vesicles and facilitate transport of
vesicles in neurons. Finally, we provide evidence that mouse brain vesicles
produce ATP from ADP and glucose, and display movement in a reconstituted in
vitro transport assay of native vesicles. We conclude that transport of vesicles
along microtubules can be autonomous.

DOI: 10.1038/ncomms13233
PMCID: PMC5078996
PMID: 27775035


Auteurs Bordeaux Neurocampus