Rhodamine phalloidin F-actin. Critical concentration versus tensile strength

Raffaella Adami, Daniel Choquet, Enrico Grazi
Eur J Biochem. 1999-07-01; 263(1): 270-275
DOI: 10.1046/j.1432-1327.1999.00501.x

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1. Eur J Biochem. 1999 Jul;263(1):270-5.

Rhodamine phalloidin F-actin: critical concentration versus tensile strength.

Adami R(1), Choquet D, Grazi E.

Author information:
(1)Dipartimento di Biochimica e Biologia Molecalare, Universitá di Ferrara,
Italy.

The mechanic and elastic properties of rhodamine phalloidin F-actin were
investigated as a function of the ionic strength and in the absence of Mg2+. By
increasing ionic strength from 3 to 19 mM, critical concentration decreased from
146 to 36 nM and the yield strength increased from 5.6 pN to 28.6 pN. At the
ionic strength of 12-13 mM, the elastic modulus by stretching increased by
330-430 kP. nm-1 up to the break point, where it was 38-44.2 MP. The work
required to break the filament, 403-439 kJ.M-1 provides an estimate of the free
energy of annealing of rhodamine phalloidin F-actin, the annealing constant being
2.8 x 1074 M-1.

DOI: 10.1046/j.1432-1327.1999.00501.x
PMID: 10429213 [Indexed for MEDLINE]

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