Regulation of AMPA receptor lateral movements
Nature. 2002-06-01; 417(6889): 649-653
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1. Nature. 2002 Jun 6;417(6889):649-53.
Regulation of AMPA receptor lateral movements.
Borgdorff AJ(1), Choquet D.
(1)CNRS UMR 5091, Université de Bordeaux 2, Bordeaux 33077, France.
Nature. 2002 Jun 6;417(6889):601-2.
An essential feature in the modulation of the efficacy of synaptic transmission
is rapid changes in the number of AMPA
(alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid) receptors at
post-synaptic sites on neurons. Regulation of receptor endo- and exocytosis has
been shown to be involved in this process. Whether regulated lateral diffusion of
receptors in the plasma membrane also participates in receptor exchange to and
from post-synaptic sites remains unknown. We analysed the lateral mobility of
native AMPA receptors containing the glutamate receptor subunit GluR2 in rat
cultured hippocampal neurons, using single-particle tracking and video
microscopy. Here we show that AMPA receptors alternate within seconds between
rapid diffusive and stationary behaviour. During maturation of neurons,
stationary periods increase in frequency and length, often in spatial correlation
with synaptic sites. Raising intracellular calcium, a central element in synaptic
plasticity, triggers rapid receptor immobilization and local accumulation on the
neuronal surface. We suggest that calcium influx prevents AMPA receptors from
diffusing, and that lateral receptor diffusion to and from synaptic sites acts in
the rapid and controlled regulation of receptor numbers at synapses.
PMID: 12050666 [Indexed for MEDLINE]