Red blood cell plasmalogens and docosahexaenoic acid are independently reduced in primary open-angle glaucoma
Experimental Eye Research. 2009-12-01; 89(6): 840-853
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1. Exp Eye Res. 2009 Dec;89(6):840-53. doi: 10.1016/j.exer.2009.07.008. Epub 2009
Red blood cell plasmalogens and docosahexaenoic acid are independently reduced in
primary open-angle glaucoma.
Acar N(1), Berdeaux O, Juaneda P, Grégoire S, Cabaret S, Joffre C,
Creuzot-Garcher CP, Bretillon L, Bron AM.
(1)Eye and Nutrition Research Group, UMR FLAVIC, INRA, Dijon, France.
Among several theories involved in the pathogenesis of primary open-angle
glaucoma (POAG), the vascular theory considers the disease to be a consequence of
reduced ocular blood flow associated with red blood cell abnormalities. Red blood
cell membrane structure and function are influenced by their phospholipid
composition. We investigated whether specific lipid entities that may affect the
membrane physiology, namely, polyunsaturated fatty acids (PUFAs) and
plasmalogens, are modified in POAG and whether these potential variations are
related to the stage of glaucoma. Blood samples were collected from 31 POAG
patients and 10 healthy individuals. The stage of glaucoma was determined
according to the Hodapp and Parrish classification. Lipids were extracted from
red blood cell membranes and individual phospholipid species were quantified by
liquid chromatography combined with mass spectrometry using triple quadrupole
technology. POAG patients had reduced erythrocyte levels of phosphatidyl-choline
(PC) carrying docosahexaenoic acid (DHA). POAG patients also displayed lower
levels of choline plasmalogens (PlsC) carrying PUFAs other than DHA. These
differences were greater as the severity of the disease increased. Linear
regressions predicted that red blood cell PlsC levels would decrease years before
clinical symptoms, whereas the levels of PC carrying DHA were linearly correlated
to visual field loss. Our data demonstrate the selective loss of some individual
phospholipid species in red blood cell membranes, which may partly explain their
loss of flexibility in POAG.
PMID: 19627986 [Indexed for MEDLINE]