Protective effect of green tea polyphenols on the SH-SY5Y cells against 6-OHDA induced apoptosis through ROS-NO pathway.
Free Radical Biology and Medicine. 2005-09-01; 39(5): 682-695
DOI: 10.1016/j.freeradbiomed.2005.04.022
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1. Free Radic Biol Med. 2005 Sep 1;39(5):682-95.
Protective effect of green tea polyphenols on the SH-SY5Y cells against 6-OHDA
induced apoptosis through ROS-NO pathway.
Guo S(1), Bezard E, Zhao B.
Author information:
(1)Laboratory of Visual Information processing, Center of Brain & Cognitive
Science, Institute of Biophysics, Academia Sinica, Beijing 100101, People’s
Republic of China.
Green tea polyphenols (GTP) are thought to help prevent oxidative stress-related
diseases, such as cancer, cardiovascular disease, neurodegenerative disease, and
aging. We here investigate the protective mechanisms of GTP on SH-SY5Y cells
against apoptosis induced by the pro-parkinsonian neurotoxin 6-hydroxydopamine
(6-OHDA). GTP rescued the changes in condensed nuclear and apoptotic bodies,
attenuated 6-OHDA-induced early apoptosis, prevented the decrease in
mitochondrial membrane potential, and suppressed accumulation of reactive oxygen
species (ROS) and of intracellular free Ca(2+). GTP also counteracted the
6-OHDA-induced nitric oxide increase and overexpression of nNOS and iNOS, and
decreased the level of protein-bound 3-nitrotyrosine (3-NT). In addition, GTP
inhibited the autooxidation of 6-OHDA and scavenged oxygen free radicals in a
dose- and time-dependent manner. Our results show that the protective effects of
GTP on SH-SY5Y cells are mediated, at least in part, by controlling the ROS-NO
pathway.
DOI: 10.1016/j.freeradbiomed.2005.04.022
PMID: 16085186 [Indexed for MEDLINE]