Probing microtubules polarity in mitotic spindles in situ using Interferometric Second Harmonic Generation Microscopy
Sci Rep. 2017-07-28; 7(1):
DOI: 10.1038/s41598-017-06648-4
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1. Sci Rep. 2017 Jul 28;7(1):6758. doi: 10.1038/s41598-017-06648-4.
Probing microtubules polarity in mitotic spindles in situ using Interferometric
Second Harmonic Generation Microscopy.
Bancelin S(1), Couture CA(1), Pinsard M(1), Rivard M(1), Drapeau P(2), Légaré
F(3).
Author information:
(1)Institut National de la Recherche Scientifique, Centre Energie Matériaux
Télécommunications (INRS-EMT), Université du Québec, 1650 boulevard
Lionel-Boulet, Varennes, QC, J3X 1S2, Canada.
(2)Département de Neurosciences, Centre de Recherche, Centre Hospitalier de
l’Université de Montréal, Montreal (QC), 900 rue Saint-Denis, Pavillon R, H2X
0A9, Canada.
(3)Institut National de la Recherche Scientifique, Centre Energie Matériaux
Télécommunications (INRS-EMT), Université du Québec, 1650 boulevard
Lionel-Boulet, Varennes, QC, J3X 1S2, Canada. .
The polarity of microtubules is thought to be involved in spindle assembly,
cytokinesis or active molecular transport. However, its exact role remains poorly
understood, mainly because of the challenge to measure microtubule polarity in
intact cells. We report here the use of fast Interferometric Second Harmonic
Generation microscopy to study the polarity of microtubules forming the mitotic
spindles in a zebrafish embryo. This technique provides a powerful tool to study
mitotic spindle formation and may be directly transferable for investigating the
kinetics and function of microtubule polarity in other aspects of subcellular
motility or in native tissues.
DOI: 10.1038/s41598-017-06648-4
PMCID: PMC5533768
PMID: 28754928