Primary cilium migration depends on G-protein signalling control of subapical cytoskeleton

Jerome Ezan, Léa Lasvaux, Aysegul Gezer, Ana Novakovic, Helen May-Simera, Edwige Belotti, Anne-Catherine Lhoumeau, Lutz Birnbaumer, Sandra Beer-Hammer, Jean-Paul Borg, André Le Bivic, Bernd Nürnberg, Nathalie Sans, Mireille Montcouquiol
Nat Cell Biol. 2013-08-11; 15(9): 1107-1115
DOI: 10.1038/ncb2819

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1. Nat Cell Biol. 2013 Sep;15(9):1107-15. doi: 10.1038/ncb2819. Epub 2013 Aug 11.

Primary cilium migration depends on G-protein signalling control of subapical
cytoskeleton.

Ezan J(1), Lasvaux L, Gezer A, Novakovic A, May-Simera H, Belotti E, Lhoumeau AC,
Birnbaumer L, Beer-Hammer S, Borg JP, Le Bivic A, Nürnberg B, Sans N,
Montcouquiol M.

Author information:
(1)1] INSERM, Planar Polarity and Plasticity Group, Neurocentre Magendie, 33077
Bordeaux, France [2] Université Bordeaux, Neurocentre Magendie, Bordeaux 33077,
France.

In ciliated mammalian cells, the precise migration of the primary cilium at the
apical surface of the cells, also referred to as translational polarity, defines
planar cell polarity (PCP) in very early stages. Recent research has revealed a
co-dependence between planar polarization of some cell types and cilium
positioning at the surface of cells. This important role of the primary cilium in
mammalian cells is in contrast with its absence from Drosophila melanogaster PCP
establishment. Here, we show that deletion of GTP-binding protein alpha-i subunit
3 (Gαi3) and mammalian Partner of inscuteable (mPins) disrupts the migration of
the kinocilium at the surface of cochlear hair cells and affects hair bundle
orientation and shape. Inhibition of G-protein function in vitro leads to
kinocilium migration defects, PCP phenotype and abnormal hair bundle morphology.
We show that Gαi3/mPins are expressed in an apical and distal asymmetrical
domain, which is opposite and complementary to an aPKC/Par-3/Par-6b expression
domain, and non-overlapping with the core PCP protein Vangl2. Thus
G-protein-dependent signalling controls the migration of the cilium cell
autonomously, whereas core PCP signalling controls long-range tissue PCP.

DOI: 10.1038/ncb2819
PMID: 23934215 [Indexed for MEDLINE]


Auteurs Bordeaux Neurocampus