Potassium channel expression level is dependent on the proliferation state in the GH3 pituitary cell line.
American Journal of Physiology-Cell Physiology. 2003-04-01; 284(4): C1054-C1064
DOI: 10.1152/ajpcell.00446.2002
Lire sur PubMed
Previously, we showed that the peak density of the transient outward K+ current ( I to) expressed in GH3 cells was different in the S phase than in other phases of the cell cycle. Using cell synchronization, we show here that I to drops precisely at the quiescent (G0 phase)/proliferating transition. This change is not due to a modification in the voltage dependence of I to, but rather to a modification in its inactivation kinetics. Molecular determination of K+channel subunits showed that I to required the expression of Kv1.4, Kv4.1, and Kv4.3. We found that the increase in I to density during the quiescent state was accompanied by an increase in Kv1.4 protein expression, whereas Kv4.3 expression remained unchanged. We further demonstrate that the link between I to expression and cell proliferation is not mediated by variations in cell excitability. These results provide new evidence for the cell cycle dependence of I to expression, which could be relevant in understanding the mechanisms leading to pituitary adenomas.