P2X Electrophysiology and Surface Trafficking in Xenopus Oocytes

Eléonore Bertin, Audrey Martínez, Eric Boué-Grabot
Methods in Molecular Biology. 2019-10-24; : 243-259
DOI: 10.1007/978-1-4939-9717-6_18

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Xenopus oocytes serve as a standard heterologous expression system for the study of various ligand-gated ion channels including ATP P2X receptors. Here we describe the whole-cell two-electrode voltage clamp and biotinylation/Western blotting techniques to investigate the functional properties and surface trafficking from P2X-expressing oocytes.


Auteurs Bordeaux Neurocampus