Opposite change of in vivo dopamine release in the rat nucleus accumbens and striatum that follows electrical stimulation of dorsal raphe nucleus: Role of 5-HT3 receptors
J. Neurosci.. 1998-08-15; 18(16): 6528-6538
DOI: 10.1523/JNEUROSCI.18-16-06528.1998
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1. J Neurosci. 1998 Aug 15;18(16):6528-38. doi:
10.1523/JNEUROSCI.18-16-06528.1998.
Opposite change of in vivo dopamine release in the rat nucleus accumbens and
striatum that follows electrical stimulation of dorsal raphe nucleus: role of
5-HT3 receptors.
De Deurwaerdère P(1), Stinus L, Spampinato U.
Author information:
(1)Institut National de la Santé et de la Recherche Médicale Unité 259, Unité
Mixte de Recherche-Centre National de la Recherche Scientifique 5541, Université
Victor Ségalen Bordeaux 2, 33077 Bordeaux Cedex, France.
In the present study we investigate, using in vivo microdialysis, the
involvement of central 5-HT3 receptors in the effect of dorsal raphe nucleus
(DRN) electrical stimulation on dopamine (DA), 3, 4-dihydroxyphenylacetic acid
(DOPAC), and 5-hydroxyindole-3-acetic acid (5-HIAA) extracellular levels
monitored in the nucleus accumbens and the striatum of halothane-anesthetized
rats. DRN stimulation (300 microA, 1 msec at 3, 5, 10, and 20 Hz for 15 min)
induced a frequency-dependent increase of accumbal DA release and a concomitant
reduction of DA release in the ipsilateral striatum at 20 Hz. In both structures
DOPAC and 5-HIAA dialysate contents were enhanced in a frequency-dependent
manner. Central serotonin (5-HT) depletion, induced by intra-raphe injections of
5, 7-dihydroxytryptamine neurotoxin, abolished the effect of 20 Hz DRN
stimulation on DA, DOPAC, and 5-HIAA extracellular levels in both regions. The
5-HT synthesis inhibitor para-chlorophenylalanine (3 x 400 mg/kg, i.p., for 3
d), although preventing the effect on DA release, failed to modify significantly
the effect of 20 Hz DRN stimulation on DOPAC and 5-HIAA outflow in both
structures. Ondansetron (0.1 and 1 mg/kg) and (S)-zacopride (0.1 mg/kg), two
5-HT3 antagonists, significantly impaired the increase of accumbal DA release
induced by 20 Hz DRN stimulation but did not affect either the decrease of
striatal DA release or the increase in DOPAC outflow in both structures. These
results indicate that an enhancement of central 5-HT transmission induced by DRN
stimulation differentially affects striatal and accumbal DA release and that
endogenous 5-HT, via its action on 5-HT3 receptors, exerts a facilitatory
control restricted to the mesoaccumbal DA pathway.
DOI: 10.1523/JNEUROSCI.18-16-06528.1998
PMCID: PMC6793211
PMID: 9698340 [Indexed for MEDLINE]