Neuropeptide RNA localization in tissue sections
Methods in Molecular Biology. 2011-01-01; : 73-87
DOI: 10.1007/978-1-61779-310-3_4
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1. Methods Mol Biol. 2011;789:73-87. doi: 10.1007/978-1-61779-310-3_4.
Neuropeptide RNA localization in tissue sections.
Landry M(1), Salam SA, Moftah M.
Author information:
(1)INSERM U862, University of Bordeaux, Bordeaux, France.
In situ hybridization has become a routine technique to provide insights into RNA
localization. However, different protocols exist for multiple purposes, and it
is, therefore, important to clearly define specific needs to choose the most
suitable one(s). For instance, in situ hybridization can target different types
of RNA, including mRNA or small noncoding RNA such as micro RNA (miRNA).
Detection protocols are developed for light or electron microscopy and can be
combined with immunocytochemistry to study RNA coexpression with proteins or
peptides. In this chapter, we present some protocols to illustrate the diversity
of in situ hybridization methods. We focus on the detection of mRNA or miRNA and
show that the protocols are quite similar but use dedicated probe types, namely,
oligo- or riboprobes and locked nucleic-acid probes.
DOI: 10.1007/978-1-61779-310-3_4
PMID: 21922401 [Indexed for MEDLINE]