Neither the density nor function of striatal dopamine transporters were influenced by chronic n-3 polyunsaturated fatty acid deficiency in rodents
Neuroscience Letters. 2002-03-01; 321(1-2): 95-99
DOI: 10.1016/s0304-3940(01)02481-8
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Kodas E(1), Page G, Zimmer L, Vancassel S, Guilloteau D, Durand G, Chalon S.
Author information:
(1)INSERM U316, Laboratoire de Biophysique Médicale et Pharmaceutique,
Université François Rabelais, UFR des Sciences Pharmaceutiques, 31 Avenue Monge,
37200 Tours, France.
We hypothesized that the chronic dietary deficiency of n-3 polyunsaturated fatty
acids (n-3 PUFAs) might affect the density and/or function of dopamine
transporters (DAT), which have a major role in regulating the synaptic level of
dopamine. This hypothesis was tested by investigating DAT in the striatum using
three complementary methods in control and deficient rats. The density of DAT
was determined by quantitative autoradiography using [(125)I]PE2I, a specific
ligand of this transporter. Functional investigations were performed (i) in
vitro by measuring [(3)H]dopamine uptake on synaptosomes, and (ii) in vivo using
intracerebral microdialysis. The results demonstrated that neither the density
nor the function of DAT were influenced by n-3 PUFA deficiency in the striatum.
This suggests lower sensitivity to n-3 PUFA deficiency in the striatum than that
previously observed in the frontal cortex.