Near-Infrared Carbon Nanotube Tracking Reveals the Nanoscale Extracellular Space around Synapses

Chiara Paviolo, Joana S. Ferreira, Antony Lee, Daniel Hunter, Ivo Calaresu, Somen Nandi, Laurent Groc, Laurent Cognet
Nano Lett.. 2022-08-29; :
DOI: 10.1021/acs.nanolett.1c04259

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Paviolo C(1), Ferreira JS(2), Lee A(1), Hunter D(2), Calaresu I(2), Nandi S(1), Groc L(2), Cognet L(1).

Author information:
(1)Université de Bordeaux, Institut d’Optique & Centre National de la Recherche Scientifique, UMR 5298, 33400 Talence, France.
(2)Université de Bordeaux, Interdisciplinary Institute for Neuroscience, UMR 5297, 33076 Bordeaux, France.

We provide evidence of a local synaptic nanoenvironment in the brain extracellular space (ECS) lying within 500 nm of postsynaptic densities. To reveal this brain compartment, we developed a correlative imaging approach dedicated to thick brain tissue based on single-particle tracking of individual fluorescent single wall carbon nanotubes (SWCNTs) in living samples and on speckle-based HiLo microscopy of synaptic labels. We show that the extracellular space around synapses bears specific properties in terms of morphology at the nanoscale and inner diffusivity. We finally show that the ECS juxta-synaptic region changes its diffusion parameters in response to neuronal activity, indicating that this nanoenvironment might play a role in the regulation of brain activity.

Auteurs Bordeaux Neurocampus