Migration and differentiation of human mesenchymal stem cells in the normal rat brain.

Jia-Mei Li, Hua Zhu, Shan Lu, Ying Liu, Qin Li, Paula Ravenscroft, Yan-Feng Xu, Lan Huang, Chun-Mei Ma, Erwan Bezard, Robert Chun-Hua Zhao, Ren-zhi Wang, Chuan Qin
Neurological Research. 2011-01-01; 33(1): 84-92
DOI: 10.1179/016164110x12670144737819

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1. Neurol Res. 2011 Jan;33(1):84-92. doi: 10.1179/016164110X12670144737819. Epub
2010 May 19.

Migration and differentiation of human mesenchymal stem cells in the normal rat
brain.

Li JM(1), Zhu H, Lu S, Liu Y, Li Q, Ravenscroft P, Xu YF, Huang L, Ma CM, Bezard
E, Zhao RC, Wang RZ, Qin C.

Author information:
(1)Department of Pathology, Institute of Laboratory Animal Science, Chinese
Academy of Medical Sciences and Peking Union Medical College, China.

OBJECTIVES: Transplanted mesenchymal stem cells migrate toward brain lesions and
differentiate into neurons, glial cells, and neural stem cells in diseased or
injured animal models. The migratory routes and differentiation patterns of
mesenchymal stem cells in normal rats are, however, unknown. Here, labelled human
mesenchymal stem cells (or saline) were transplanted into the striatum of adult
rats to observe their migration and differentiation.
METHODS: Labelled human mesenchymal stem cells were transplanted into the right
striatum of adults rats (n = 24). Brain sections were examined for migratory
routes of labelled human mesenchymal stem cells by immunohistochemistry method,
fluorescence microscope and laser scanning confocal microscopy observation, and
Prussian blue staining. Moreover, the differentiation of human mesenchymal stem
cells was detected by double immunohistochemistry.
RESULTS: After 3 days, most human mesenchymal stem cells resided around the
injection sites. Human mesenchymal stem cells were found in or around the corpus
callosum and the subependymal layer after 7 days. A great number of human
mesenchymal stem cells were detected throughout the brain on both ipsilateral and
contralateral sides after 14 days. A high concentration of donor cells persisted
in the corpus callosum, the external capsule and the subventricular zone. In
addition, the incorporated human mesenchymal stem cells were neuronal nuclei- and
glial fibrillary acidic protein-positive.
CONCLUSION: Human mesenchymal stem cells migrate throughout the brain mainly
along with the axis of corpus callosum external capsule and the subependymal
layer, and differentiate into neurons and astrocytes rather than neural stem
cells.

DOI: 10.1179/016164110X12670144737819
PMID: 20487599 [Indexed for MEDLINE]

Auteurs Bordeaux Neurocampus